![the intensities of bands were determined by imagej software the intensities of bands were determined by imagej software](https://www.researchgate.net/profile/Amanda-Lewis-13/publication/232308684/figure/fig5/AS:269833963044876@1441344838830/Secretory-IgA-is-more-protease-resistant-than-IgG-Human-antibodies-were-tested-for.png)
When we capture a picture and transform it to a digital image, we have to distribute the information across our states. Now let’s try to think about bit depth from another standpoint. The number of combinations is equal to 2^n where n is the number of bits, in other words: the number of possible combinations grows exponentially. If we add a third bit, we can store eight different values, so we would have eight shades of gray. So if we would define an image format with two bit depth, we could display now four different shades of gray. If we have two bits instead, we can store even more combinations: bits Conversely, every pixel can only store two different combinations - black (0) or white (1). Since the information of every pixel is stored in one single bit, our simple image format has a one bit depth. This version is obviously scaled up and shows four gigantic pixels.Īs you can see, the 1 represents “white” and the 0 represents “black”. The positions are zero based (the first number is zero) as often the case in computer science. Try to figure out what the 0 and 1 represents. The following table could for instance create the image below (the displayed image is scaled up). Every pixel stores information about the color at that place. What is an imageĪ digital image is in its simples form an arrangement of pixels.
The intensities of bands were determined by imagej software how to#
We can see two things: a) we need to transform every information into combinations of 1 and 0, in order to process this information digitally b) we need to define certain rules how to interpret data. But it could also mean the decimal number 18537. Similarly, the values 0100100001101001 could either be interpreted as Hi, if you treat it as text. And suddenly 562 has a different meaning. It could also be the extension of my phone number. But if I would tell you, that this is an office number, the 562 suddenly has a meaning. Without context, you can’t really do anything with 562. The actual information is received, when interpreting these bits in a certain way.
![the intensities of bands were determined by imagej software the intensities of bands were determined by imagej software](https://www.researchgate.net/publication/326962744/figure/fig5/AS:658331676966912@1533969911048/Gelatin-degradation-by-MMP9-produced-in-MCF-7-MMP9-cells-A-Band-intensity_Q640.jpg)
Everything that is processed has to be represented by a chain of zeros and ones - a combination of bits. A bit is a storage that can either have the state 1 or 0 and is the smallest digital unit. Further improvement of reagents and assay formats is necessary to reduce the high background problem in this study.Your computer actually knows only two states: 0 and 1. This condition was demonstrated on the membrane-based immuno-PCR procedure to detected human IL-6 and reported the detection sensitivity is <0.001 pg/ml of human IL-6. Premixing of the detector antibody and DNA, 5 minutes waiting time, and 0.5 mg/ml spraying concentration of capture antibody were concluded the best condition to give the best signal.
![the intensities of bands were determined by imagej software the intensities of bands were determined by imagej software](https://www.researchgate.net/profile/Iain-Campbell/publication/7082525/figure/fig2/AS:280360085671947@1443854461476/Analysis-of-chemokine-mRNA-expression-a-RPA-gel-showing-bands-corresponding-to-mRNAs.png)
The 1:1 ratio of antibody to DNA-streptavidin complex was found to give the best result and therefore selected for further test. Two methodologies were tested the effect of the ratio of DNA to streptavidin and the ratio antibody to DNA-streptavidin complex. The detector antibody was optimized its conjugation method for the DNA-streptavidin complex. The DNA reporter was amplified by PCR to enhance the detection sensitivity. But instead the gold nanoparticles-conjugated antibody, this study employed DNA-conjugated antibody as the signal reporter. The test procedure followed the traditional lateral flow immunochromatography assay. Membrane-based immuno-PCR procedure was developed by applying the immuno-PCR technique on membrane nitrocellulose to increase the sensitivity of the lateral flow immunochromatography assay.